Auditory and Visual free essay sample

If a variety of people are put through numerous trials of visual memory tests and auditory tests, then the people with better auditory memory will outnumber the people with better visual memory. Introduction Visual and auditory memory play vital roles in learning and development. They are both skills required to have throughout life. By definition, auditory memory is the ability to process information presented orally, analyze it, and store it to be recalled later. Visual memory by definition is the ability to process information presented visually, analyze it mentally, and remember it for a later time. Both of these skills are step-by-step developmental processes. They continue to grow when a person is growing themselves. Auditory memory is the ability to process information that is presented orally, analyze it mentally and store it to be remembered later. To be an auditory learner is to have a strong capacity for auditory memory. Auditory memory is also the ability to learn from instructions that were presented orally; it is an important skill that will help in life. We will write a custom essay sample on Auditory and Visual or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Auditory memory has to be one of the most important skills in learning. Some children who show a delayed grasp of language have weak auditory learning skills, they also have difficulty understanding words. Parents can test their child’s auditory memory by saying a sequence of numbers and asking the child to repeat the numbers back to them. Through exercises auditory memory skills can be developed. Children as well as adults can sharpen their auditory memory skills. Visual memory is the ability to psychologically recall visual images in the form of objects, events, or words. Students with disabilities have a serious deficiency with visual memory. Storing and retrieving previously experienced visual sensations and perceptions when stimuli that were evoked originally are no longer present are involved in visual memory. Many researchers have stated that 80% of learning takes place through the eye with visual memory. Visual memory is crucial in the aspect of learning. If a person cannot adequately reproduce a sequence of stimuli, then they may not have developed their visual memory skills. People who have difficulty with visual memory have trouble overall remembering the visual appearance of words, a letter sequence of words, and spelling. When people have trouble with visual memory can often remember the letters in a word but not the sequence. Some people with serious writing and spelling difficulties have trouble with their visual memory skills. The differences are that auditory memory works with the ears auditorally and visual memory works with the eyes visually. To be an auditory learner is to have a strong capacity for auditory memory. Visual memory is the ability to psychologically recall visual images in the form of objects, events, or words. Auditory memory has to be one of the most important skills in learning. Visual memory is crucial in the aspect of learning. When people have trouble with visual memory can often remember the letters in a word but not the sequence. Auditory memory is also the ability to learn from instructions that were presented orally. Parents can test their child’s auditory memory. Caffeine can affect memorization by increasing it. The ability to remember is a basic but important function that is critical to survival today. Memory is especially important for students who live their lives learning. Age can also affect memorization ability. Studies have shown that staying active can help stabilize memory change when it comes to aging. There are a few ways to positively affect memory change; they include physical activity, mental activity and a healthy diet. The primary factor affecting a person’s memorization change is a medical condition. There are a few medical factors that can negatively affect memory change; medical disorders, diseases, emotional problems, medication, medical changes, and a poor diet. Crossword puzzles can help positively affect memorization. Hormonal changes can affect memorization. Adults are twice as likely to lose mental capacity. Many body parts are involved in auditory and visual memory. The posterior parietal cortex is a portion of the parietal lobe in the brain. Activity in the posterior parietal cortex is extremely correlated with the information that can be stored in visual memory. This suggests that the posterior parietal cortex is vital in our visual representation of the world. It acts as a limited storage area for everything we see. Another important body part involved in visual memory are the occipital lobes. They are located at the back of the brain. They receive and process information. The lobes tend to process colors and shapes. They are responsible for identifying colors while visually memorizing objects. Body parts involved in auditory memory are slightly different. Auditory sensory memory tends to be stored in the primary auditory cortex closer to the ear of presentation. However, auditory memory involves many different brain parts. The majority of brain regions involved in auditory memory are located in the prefrontal cortex. This is where the executive control is located, and is responsible for attention control. Brain areas are actually a major factor in understanding why some ways of memorizing things work better than other ways. Visual memory tends to be the most readily available to us. When your brain receives information, it begins to decide what it and isn’t important. It also begins to store it as long-term. Neural connections become more powerful as the information is received and is connected to emotions. Neurons are nerve cells, and a pattern of connections between them forms a memory. Seeing something familiar tends to have a greater impact on the mind than hearing something familiar. The brain can form new neural pathways and alter existing connections. It is very easy to improve visual and auditory memory. If one organizes and structures the information they are trying to memorize, it will help immensely. It is important to visualize concepts to improve memory. Charts, graphs, and photos are all great things to look upon when memorizing. Numerous research studies have been done on visual and auditory memory. Much effort has been dedicated to investigating the capacity limits of memory in the brain. Steven J. Luck and Edward K. Vogel are two men from the University of Iowa who are known for their research on this topic. They sorted memory into two categories- long term and short term. These findings are foundation for all different types of memory. Experimental Procedure 1. In this experiment, number sequences will be needed for the test subjects to remember. Each number sequence should be composed of the numbers 0–9 and be seven digits long. This random number generator can be used to figure out the number sequences. http://www. random. org/nform. html 2. The experimenter should fill out the form on the generator so it has 7 integers, 1 as the smallest value, 9 as the largest value, and a format in 7 columns. Then the experimenter should hit Get Numbers and a new page will appear with 7 listed numbers at the top. To get new numbers, it is not necessary to fill out the form again, so simply click the refresh button on the browser window and a new set of random numbers appears: 3. The experimenter should write one number sequence on each index card, until the deck of cards consists of about 50 different random sequences. This deck will be used for the experiment. 4. The experimenter should find 25 random research participants, and ask each test subject to take two memory tests. Then give them the two following tests: A: To test ones visual memory, show the subject a card for 30 seconds and time with a timer. Take back the card and have the subject recite the alphabet. Then ask the subject to say what the numbers were. Write down how many numbers the subject got right. This will be the subject’s score. Record the score. B: To test ones auditory memory, read the sequence of numbers on a different card three times slowly. After the numbers have been read, have the test subject recite the alphabet. Then ask the subject to say what the numbers were. Write down how many numbers the subject got right. This will be the subject’s score. Record the score. 5. Calculate the percentage of people who received each score. Do this by first adding the total number of participants for each test, then divide the number of people receiving the score by the total number of participants in the study. Multiply the answer by 100 to get the percentage. 6. Analyze the data by making a histogram. On the left side of the graph (Y-axis), write a scale for the percentage of people from 0 to 100%. On the bottom of the graph, write a scale for the number of correct responses from 0 to 7.

Research Methods and Statistics Midterm Exam Essays

Research Methods and Statistics Midterm Exam Essays Research Methods and Statistics Midterm Exam Essay Research Methods and Statistics Midterm Exam Essay In the formula n Is the sample size. Median position = (n + 2 Median position = (24 + 1)/2 Median position = 25/2 = 12. 5 Now we look for position 12 and 13. Position 12 is 5, and position 13 is 6. We take the average of the two values and that is the median. Median = (5 11/2= 5. 5 Median = 5. 5 The mode is the most frequent response in the distribution 6-1 2-2 7-2 3-6 8-6 4?2 5-1 10-1 In this example we have two modes. They are 3 and 8. A creativity test. The student scored 123 on the intelligence test and 123 on the creativity test. The mean for the intelligence test is 100, and the standard deviation is 16. The mean for the creativity test is 115, and the standard deviation is 14. What statistic would you use to compare the two scores from these two different distributions (the intelligence test distribution and the creativity test distribution)? Compute this statistic and determine on which test, if either, the student performed better. Explain your answer. (10 pets) Intelligence test score = 123, mean = 100, tankard deviation = 16 Creativity test score = 123, mean = 1 15, standard deviation = 14 To determine on which test the student did better, we use the standard deviation and the mean. To do the determination we have to use the standard deviation in proportion to the normal curve. Intelligence 129 Creativity Range: 84- 116 101 The student did better in the intelligence test since the mean for both test is within the range for the intelligence test. 3) a. Calculate the standard deviation for the following distribution: (5 pets total) calculate the mean (1+2+3+7+8+9) = 30 / 6 = 5 Subtract the mean from each score 1 -5=-4 7-5=2 2-5=-3 8-5=3 Square the resulting difference for each score. These are the squared deviations. -4 X -4 = 16 -3 x-3=9 EX.=9 -2 x-2=4 4 X 4=16 Add the squared deviations. (16+9+4 +4+9+ 16) = 58 Divide the squared deviations by the sample size to get the variance. 58 / 6 Take the square foot of the variance to get the standard deviation. Standard deviation = 3. 11 = 9. 67 -7=0 2-7=-5 8-7=1 3-7 9-7=2 Square the difference -6 x -6=36 OX=O -5 x -5=25 XIX- -4 x -4=16 ex.=4 Add the squared deviations and divide by the sample size 36 +25+16+0+1 Take the square foot of the variance = 3. 9 = 3. 7 c. What percentage of people would have scores falling between the mean and this value? 13. 66% d. What percentage of people would have scores falling at or below this value? 27. 32% 4) Sally was given a standardized test in which the mean for her class was 500, and the standard deviation was 100. Sally scored 400. Compute her score to a z-score (show your work). How did Sally perform relative to the rest of her class (be specific)? (10 pets) z = (400-500) 1100 z100/100 z

Analysis essay about parental expectations Example | Topics and Well Written Essays - 1000 words

Analysis about parental expectations - Essay Example wise, as parental expectations play an essentially vital role in the performance of a child, ridiculously high expectations can result in their failure while low expectations can lead to under confidence. Maintaining too high expectations can also result in social anxiety, stress, depression and many other forms of mental illnesses. Every child has a different learning pace; it could be fast for some while it comes slower to others. Some children may be self sufficient while others could need help guidance and tutoring. It is an accepted fact that every child has a unique learning pace which can be molded and improved with encouragement. This suggests that the same amount expectations cannot be held with every child. That said, parental involvement does make for overall better performance in children than in the case of those whose parents are less involved. Although there are many sides to parenting, studies show that the greatest influence is cast by a single aspect, parental expectations, according to the Harvard Family Research Project (Harvard 2014). In order to provide a child with a healthy home environment, it is essential to love him unconditionally, irrespective of whether or not he is the brightest of the lot. Now the thing that gets in the way of this unconditional love is our sky high expectations (Hudson 2014). An explanation for why parental expectations are so affective on children is that they see themselves, for most of their childhood and even beyond, through their parents’ eyes. Consequently, if a parent believes his child can be a top-dog, that’s what his child will perceive himself to be. On the other hand, if a child has been exposed to derogatory aura at home, he is most likely to accept failure without really trying at all. Similarly, having far greater expectations from your child than his obvious abilities is like expecting a chicken to swim or a cat to fly. The key here is for parents to analyze the situation their child is in

Inventory stystems Assignment Example | Topics and Well Written Essays - 250 words

Inventory stystems - Assignment Example The system used by Kroger allows for the shelves to look like they are always full with the same type of products. This allows the products to look readily available at all times. The system works well when the projected amount of sales are accurately made. Allowing for too much or too little inventory will mean for inadequate operating costs. This is also known as order quantity issues. This can mean losing customers or allowing food to expire. Firms like Kroger that use perishable inventory are dependent on an accurate inventory. Order quantity issues can arise from any type of inventory system. This system that is used by Kroger is pretty acceptable. The only problem is that because most of the inventory is perishable, inventory accuracy must be perfect so that money is not lost. Proper management, research and production teams are essential to making sure proper inventory levels are maintained. The development of a new hybrid model can eliminate wasted inventory. Wasted inventory is a common problem for firms such as Kroger. No matter which inventory system is used, there has to be some sort of a control that allows the firm using the system to remain orderly and profitable. Without the chance for steady inventory and profit, the firm will not survive. Inman, Anthony. "Inventory Management - Levels, System, Model, Type, Business, System, What Is Inventory?, Why Keep Inventory?, Controlling Inventory, Balancing Inventory and Costs, Other Lot-sizing Techniques." Reference For Business - Encyclopedia of Small Business, Business Biographies, Business Plans, and Encyclopedia of American Industries. Web. 06 Mar. 2011.

Ethics Essay Example for Free

Ethics Essay Businesses are concerned with their company image, breaking of the laws in work related duties and any doing that will involve a law suit against the company. This is mainly their reputation and restoring it will involve a lot of money. To avoid all of this, they adapt a moral behavior in knowing what is right and wrong for the company. Therefore ethics are derived from the moral obligations, profit maximization and in following the laws set. In human ethics, an individual is expected to prevent harm, be fair and just, respect the opinion of others, have a heart for others, to be honest and wiling to comply with the law (Pojman, 2004). In professionalism, there is need for objectivity and impartiality, avoidance of conflict of interest, confidentiality of information, duty of care respected, integrity and full disclosure of information. History of utilitarianism A proposition on utilitarianism lie with Hume but its formulation and understanding credit is received by Bentham. It was incorporated during the 18th century. It was intended to view the principle of utility and the motivation of human beings. They found out that happiness and pain were humans’ utility and that what generates more happiness to many people is the ultimate right. However, having a large number being happy was difficult thus they settled for principle of happiness to represent utilitarianism. Since then, it has been an influence to economics and used to support different political moves. Introduction Utilitarianism is a principle of moral ethics. It supports that a moral right action in a situation is the one that produces more advantages than disadvantages (Buckle, 2007). Many individuals use this type of ethics in daily decisions as it brings more benefits even if lies or manipulation of others were used. It is easily aid sin decision making process. The required courses of actions are identified then their advantages and disadvantages in value are highlighted and lastly the action that generates more advantages is then selected to be morally right. It is characterized by the relevant consequences, impartiality and neutrality of the action as every person’s happiness is counted the same. The act and rule utilitarianism are its types and have been competing on which real should be followed. In act, what benefits the people more is the right morals despite their different feelings. However, the rule type acknowledges the justice, fairness and benefits a large number of people. Therefore the rule values the people feelings, their laws and the benefits. They both have problems in comparing the exact consequences value in money and happiness since they all differ. They believe in obligation to the society and not the act of being a heroine. Since it is consequences on choice, they tend to predict the future. However, act type will be favorable to one today and may be unfavorable the next day. The rule type on the other hand, is very instable on what is actually morally right. Utilitarianism can be objective in supporting the effect that the rightness of an activity is whether it would indeed encourage and promote the goodness of the already available acts to the agents. On the other side, it can take the form of subjective utilitarianism. It involves that when choosing an activity one should first determine which activities of those available would mostly promote the good more than the others, then chose that activity. Critics against utilitarianism It is difficult t assign value in money form to certain items and acts such as human life, happiness, pain, pleasure and more feelings (Pojman, 2004). They could be representing opposition’s ideas in the sense that someone’s pleasure could be another individual’s pain. This does make the comparison of values difficult for a person to choose what action will give a high better pleasure. The benefits and costs value may be underestimated or overestimated. This principle violates the human conscious acts and the different personal ideas in interest of more valuable advantages of an action. It’s like it gives us permission to do harm but specifically little harm in order to gain. In protest then, little or more harm is still harm, it never changes. Lying for the sake of saving more benefits represents an injustice to human kind. It does not promote justice but rules in favor of injustice. It is difficult to know how right an action is when the law seconds some offences committed with the excuse of the principle of utilitarianism. It does not follow the law guide thus can at all times be interchanged to commit evil. The integrity, reputation and image of a person are lost with utilitarianism ethics. The consequences of a lie for the sake of creating more profits to a company, kills the integrity that was taught of being present. Maintenance of reputation is not easy and therefore loosing the little you have will mean a slow start in creating another image. It does not encourage creativity and development of products and services as it tends to cut out considerations of new ideas generation. To other individual this is a difference making on what and how they feel. They argue that each one is responsible for what hey do and not the value of how they do it. An individual might be creative and willing to undertake his idea a step ahead and fulfill it, may not succeed at all if the majority umber do not support his idea. Therefore, a chance of having had to grow an idea in other people no matte how good it would have been, is lost because of this system. It relies entirely on a single principle of ethics thus an action committed could either produce best or worst consequences despite the argument that it will provide more benefits. Different principles in deciding what is right or wrong have to be used on a single action so as to make the solution effective and efficient. It uses the human being as means to an end of suitable action by only considering happiness and equating it as the main pleasure of a person. By only counting happiness, it encourages individuals to do the right things for an entire wrong reason. After all, they can justify the evil doing as their happiness. The theory also concentrate on a belief that human are common in nature thus desires same things. It discourages the individual’s tastes and preferences. Some people even appreciate more things than others who do not even take an account of the thing presence. Humans are very different in personalities and their goals also differ. Grouping them a sonly one group of nature, is not justice and happiness at all. It does not take into considerations the potential intention of an individual in carrying out an action. An individual could be fulfilling his evil motives on an individual but ends up defending himself using the utility benefits thus being set free and innocent. Every action should be accounted for legally so truth of right and wrong could be identified. A person stealing money and justifying it that it was to be used for a sick person medical expenses, might b lying or not. He might be a thief in the making. Critics in support of utilitarianism In human moral life, majority value the self sacrificial factor and happiness. Utilitarianism supports these morals in their decisions as some are sacrificed and the happiness of the people affected by the consequence mattering a lot (Mayer, 2009). An individual known for self sacrificial mostly promotes peace, loyalty and unity. If an organization is willing to sacrifice some items for the entire society benefits, it is received well and brings happiness which is the goal of utilitarianism. It promotes equality, impartiality, fairness and neutrality in all its decisions (Buckle, 2007). This is because to them no one deserves special treatment at the expense of another as they are all equal. A decision is basically made from everybody’s point of view with consideration of the majority. If the majority needs a company closed down, then that percentage would be a representation that every individual of that area wants the company closed. The consideration of the outcome of an activity is important. This theory is in support of knowing the consequences first before undertaking an action. The consequences received should be of great value that would not contribute to pain but joy. These would prepare the users to face the outcome with an open mind as they had been expecting it. They could be outcomes based on researches or people personal experiences. In addition, it aids in future planning of activities leading to enhancement of efficiency. This system is straightforward and direct in evaluation because it conveniently uses one denominator in its evaluation calculations. Therefore, it becomes easy in resolving conflict interests for straightforward answers to the decision. An easy way to resolve things is many people choice as many of them understand it, support it and ready to conceptualize into their daily activities. This ensures less money and minimal time spent in evaluations process. Nietzsche on ethical concept, background and theories He explained that the existence of structure theories is as a result of leaders who dominate their decisions over others. He argues that it is through self consciousness that one can determine the evil and the good with its both doers. He did not support the utilitarianism principle of ethics and argued that it placed too much emphasis on the large numbers of people instead of an individual that his personal welfare is lost to the general crowd. In addition, his other reason was that humans do things just to survive in this world even if they were unpleasant or unhappy. Utilitarianism show there is a wrong and a right but according to him this are the terms men use to interpret a situation or experience. He believes human will be motivated by to many thing snot only pleasure, happiness and pain. Aristotle ethical concepts, background and theories He showed that conduct depended on the role of an individual habit. He said that it was in between pleasures and pain that allowed one to choose which indeed was right or wrong. Being a business minded person, he advices that the root to fairness, justice and happiness is in asking questions. He does not uses utility in his work but stresses that the behavior of a person is what is reflected on his performance and goodness, thus supporting the theory of virtue of ethics and not consequences (History of Utilitarianism, 2009). He campaigned that happiness of a person could only be known after his life and thus could not be short termed using utilitarianism. Conclusions Utilitarianism should take into account that not only making people happy is a pleasure but also involving one in the success of the project, institution, a career and a cause to ensure that they are committed. Many people though support it because it is easy to use, they have practice of using it daily and it tends to avoid the divine revelation of living morally but outside the belief in a supernatural being. A person deciding should include other principles and theories of ethics that are likely to affect an action and determine if they are right or wrong. They could use principle of; virtue, moral right, egoism, deontological, justice and fairness and their emotions reflected in their feelings. This would give a wide reflection balance of the choice picked. In all the actions an individual undertakes from a selection, he should first weigh their goodness and badness and not necessarily through its money value alone. One should be able to know how the action would affect him, the law and the society. An evil action such as killing a corrupt leader for money should not be justified that it made everyone evil that had fallen into his corrupt evils.

Comunist China And Civil Rights Violations Essay -- essays research pa

China is famous throughout history for both Tieneman square, and capitol punishment . These are each examples of human rights violations. Communist China's one child policy Is yet another example. China's one child policy was stared in 1979 as an attempt to solve their overpopulation problem. The policy states that every couple in China is allowed only one child. In order for a couple to have a child they must first have a birth coupon issued by the government before giving birth to the child. "Birth Quotas" are determined in order to have surveillance of the people who have all ready had their single child allowed to them born. The women of China must deal with their menstrual cycle being monitored publicly to stop the possibility of having a second child. They also must face pelvic examinations if they are even suspected of being impregnated. Any unauthoized pregnancies are terminated by an aboution regardless of the pregnancies progression. Graphically, The baby's head is crushed and then pulled out of the woman, just to name one of the many grusome abortion practices, killing the baby, and torturing the woman. The Chinese law has horrible effect's on the country's major population. Many Infant's are abandoned, or brutally killed at home to cut down upon expenses and fines issued by the government. In 1993 ultrasound machines were in mas importation to China, however in 1993 the use of these machines ...

Mutagen

Quantification of a Mutagen: Tobacco By Selenia Lopez November 30, 2012 Section 44 Abstract: Tobacco is commonly used and kills millions until this day. Tobacco is a potential mutagen due to all the chemicals added. The spot overlay Ames test was conducted to test at what concentration of tobacco was it at the most mutagenic. The hypothesis of this experiment was as the concentration of tobacco increases, the growth of bacteria increases. The control for this experiment had a UV positive and a UV negative. Four different tobacco concentrations, Salmonella Typhimurium of strain 1538 were incubated for 24-72 hours to observe bacterial growth.At a 100% bacterial growth was at its greatest number of colonization and at 5% the mutagen was at its least. These results reflected that tobacco has the ability to grow without histidine making it a mutagen and at which concentration was it the most mutagenic. Intro: A mutagen is a substance which increases the frequency of mutation in a plant or animal population, which can lead to a variety of consequences or alterations in the DNA structure (Ligorio, Izzotti, Pulliero, Arrigo 2011). Salmonella being a mutagen can cause mutations such as substitution, insertion, deletion and frame shift depending on the strain.S. typhimurium carries a defective gene making it unable to synthesize histidine from its culture medium. Some types of mutations can be reversed with the gene regaining its function. Tobacco having lots of chemicals with possibility of being mutagenic is known to kill an estimated six million people worldwide each year and drains $500 billion annually. It can be consumed as a pesticide and in the form of nicotine tartrate. It is sometimes used in some medicines, but most commonly used as a drug. The use of Ames test is based on the assumption that any substance that is mutagenic. For this eason the FDA uses the Ames test to screen many chemicals to measures the mutagenic strength in bacterial cells (FDA 2012). In t his experiment to test whether tobacco is mutagenic and if so at what concentration has the greatest bacterial growth. The spot overlay Ames test was conducted. Though it’s a cheap version of the Ames test, the result were still compatible. The hypothesis of this experiment was as the concentration of tobacco increases, the growth increases. The greatest growth should occur in the 100% concentration and the least in the 5% concentration. Methods and materials:The control for the experiment was an Agar plate that had a UV positive reactant with a known mutagen, and UV negative reactant that hasn’t been reactant with anything. With a micro pipette that amounted to 250ul, strain TA 1538 of Salmonella was Obtained and placed on to the Agar plate and spread with a sterilized rod. The four paper discs that have been soaked in the 5%, 25%, 50%, and 100% of tobacco concentration were placed spaced apart on the plate. The plate was sealed and place it in the incubator at 37 deg ree for 24-72 hours. Results: Table 1: The table shows the concentration of mutagens to the number of colonies observed.Concentrations | Colonies | 100% | 39 | 50% | 13 | 25% | 8 | 5% | 2 | The control resulted in the UV positive having growth and the UV negative having none at all. At a 100% bacterial growth was at its greatest number of colonization at 39. At 5% the mutagen was at its least with only 2 colonies. Discussion: The mutagenic effect of the chemical has caused many bacteria to regain the ability to grow without histidine in tobacco, causing the formation of the colonies seen around the disc (Pounikar and Dawande 2010). Not only is a tobacco a mutagen, but colony growth increased as the concentration increased.The hypothesis was supported according to the data show in the table. Even though the disc were slightly shifted. The numbers were still attainable. The 100% concentration had the highest bacterial colonization and the 5% concentration had the least as predicted. I n future experiments, more trials can be done. In order to make sure the results will remain the same every time; or using other mutagens to see how they react in the 100% concentration. This type of experiment can also be useful in finding out whether smoking tobacco or chewing tobacco is more mutagenic or if the different brands make a difference on how mutagenic they can be.Works Cited Asiatic Journal of Biotechnology Resources: Pounikar, R and Dawande, A. Y. (2010). Detection of potential carcinogens by Ames test. Doi: 01: 57-64. Department of Health Sciences: Ligorion M, Izzotti A, Pulliero A, and Arrigo P. (2011) Mutagens interfere with microRNA maturation by inhibiting DICER. An in silico biology analysis. Doi: 10. 1016 www. FDA. gov: Li Y, Yan J, Bishop M, Jones MY, Watanabe F, Biris AS, Rice P, Zhou T, Chen T. (2011) Genotoxicity evaluation of titanium dioxide nanoparticles using the Ames test and Comet assay. Mutagen Quantification of a Mutagen: Tobacco By Selenia Lopez November 30, 2012 Section 44 Abstract: Tobacco is commonly used and kills millions until this day. Tobacco is a potential mutagen due to all the chemicals added. The spot overlay Ames test was conducted to test at what concentration of tobacco was it at the most mutagenic. The hypothesis of this experiment was as the concentration of tobacco increases, the growth of bacteria increases. The control for this experiment had a UV positive and a UV negative. Four different tobacco concentrations, Salmonella Typhimurium of strain 1538 were incubated for 24-72 hours to observe bacterial growth.At a 100% bacterial growth was at its greatest number of colonization and at 5% the mutagen was at its least. These results reflected that tobacco has the ability to grow without histidine making it a mutagen and at which concentration was it the most mutagenic. Intro: A mutagen is a substance which increases the frequency of mutation in a plant or animal population, which can lead to a variety of consequences or alterations in the DNA structure (Ligorio, Izzotti, Pulliero, Arrigo 2011). Salmonella being a mutagen can cause mutations such as substitution, insertion, deletion and frame shift depending on the strain.S. typhimurium carries a defective gene making it unable to synthesize histidine from its culture medium. Some types of mutations can be reversed with the gene regaining its function. Tobacco having lots of chemicals with possibility of being mutagenic is known to kill an estimated six million people worldwide each year and drains $500 billion annually. It can be consumed as a pesticide and in the form of nicotine tartrate. It is sometimes used in some medicines, but most commonly used as a drug. The use of Ames test is based on the assumption that any substance that is mutagenic. For this eason the FDA uses the Ames test to screen many chemicals to measures the mutagenic strength in bacterial cells (FDA 2012). In t his experiment to test whether tobacco is mutagenic and if so at what concentration has the greatest bacterial growth. The spot overlay Ames test was conducted. Though it’s a cheap version of the Ames test, the result were still compatible. The hypothesis of this experiment was as the concentration of tobacco increases, the growth increases. The greatest growth should occur in the 100% concentration and the least in the 5% concentration. Methods and materials:The control for the experiment was an Agar plate that had a UV positive reactant with a known mutagen, and UV negative reactant that hasn’t been reactant with anything. With a micro pipette that amounted to 250ul, strain TA 1538 of Salmonella was Obtained and placed on to the Agar plate and spread with a sterilized rod. The four paper discs that have been soaked in the 5%, 25%, 50%, and 100% of tobacco concentration were placed spaced apart on the plate. The plate was sealed and place it in the incubator at 37 deg ree for 24-72 hours. Results: Table 1: The table shows the concentration of mutagens to the number of colonies observed.Concentrations | Colonies | 100% | 39 | 50% | 13 | 25% | 8 | 5% | 2 | The control resulted in the UV positive having growth and the UV negative having none at all. At a 100% bacterial growth was at its greatest number of colonization at 39. At 5% the mutagen was at its least with only 2 colonies. Discussion: The mutagenic effect of the chemical has caused many bacteria to regain the ability to grow without histidine in tobacco, causing the formation of the colonies seen around the disc (Pounikar and Dawande 2010). Not only is a tobacco a mutagen, but colony growth increased as the concentration increased.The hypothesis was supported according to the data show in the table. Even though the disc were slightly shifted. The numbers were still attainable. The 100% concentration had the highest bacterial colonization and the 5% concentration had the least as predicted. I n future experiments, more trials can be done. In order to make sure the results will remain the same every time; or using other mutagens to see how they react in the 100% concentration. This type of experiment can also be useful in finding out whether smoking tobacco or chewing tobacco is more mutagenic or if the different brands make a difference on how mutagenic they can be.Works Cited Asiatic Journal of Biotechnology Resources: Pounikar, R and Dawande, A. Y. (2010). Detection of potential carcinogens by Ames test. Doi: 01: 57-64. Department of Health Sciences: Ligorion M, Izzotti A, Pulliero A, and Arrigo P. (2011) Mutagens interfere with microRNA maturation by inhibiting DICER. An in silico biology analysis. Doi: 10. 1016 www. FDA. gov: Li Y, Yan J, Bishop M, Jones MY, Watanabe F, Biris AS, Rice P, Zhou T, Chen T. (2011) Genotoxicity evaluation of titanium dioxide nanoparticles using the Ames test and Comet assay.